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1- Free GSH=DTNB+Sample+Substrate 2- Total GSH=DTNB+Sample+Substrate+ NADPH+glutathione reductase 3- GSSG=Total GSH-Free GSH Another method to measure GSSG separately, is to remove the free GSH from DTNB reaction process by modification of free GSH with N-ethylmaleimide (NEM) or vinylpyridine prior to conducting assay

[PubMed] [Google Scholar] 48.Shah D, Kiran R, Wanchu A, Bhatnagar A

If the answer is less than required then probably the best thing to do is ignore whatever it is

Unlike endogenous antioxidants, synthetic supplements, such as vitamin C pills, may disrupt redox signaling by non-specifically scavenging ROS, including beneficial low-level oxidants that play a role in metabolic regulation (Forman and Zhang, 2023)