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Also in 2005, the mRNA-sequence of the equine psoriasin (a calcium-binding protein of the S100-protein family with antimicrobial activities [38]) was described and the gene was mapped to equine chromosome 5 [39]

The membranes were then washed several times with water and TBS (PBS, 0.05% Tween-20), a 1 :5000 dilution of horseradish-peroxidase conjugated goat anti-rabbit antiserum was added, and the membranes further incubated for 1 h at room temperature

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